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The TS 310 tubing set contains sixteen luer locks that are glued during the production process and must not be checked. The luer locks that are glued during the production process can be found at the pre-column, separation column, and above and below the epidural filter on the reagent vial adapter lines. Do not attempt to tighten these connections. Rotation may compromise the seal’s integrity. If manipulated and the glued seal is cracked, the integrity of the tubing set can be compromised.