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Failure to disinfect the port prior to spiking the buffer bag increases the risk of contamination. A spike that is not fully inserted and a slight twist is not applied at the end may lead to leakage or loss of sterility, compromising the process. Always ensure connections remain sterile, spikes are fully inserted, and a slight twist is applied to properly secure the spike into the buffer bag. Proper spiking of the buffer bags is essential to prevent leakage, maintain sterility, and ensure con