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The tubing from the Filtration Bag will be installed into valves 1 and 23. During the process, cells are transferred from the chamber to this bag for filtration. The bottom line of the Filtration Bag should be inserted into valve 1 and the top line into valve 23. Adjust the height of Bag Hanger A. If the tubing from valve 23 becomes tight, gently pull down the tubing loop at the top of the Filtration Bag to create additional slack. This will allow you to raise the hanger higher so the bubbl